Nanotopographical induction of osteogenesis through adhesion, bone morphogenic protein co-signaling and regulation of microRNAs

Yang, J., McNamara, L. E., Gadegaard, N. , Alakpa, E. V., Burgess, K. V., Meek, D. and Dalby, M. J. (2014) Nanotopographical induction of osteogenesis through adhesion, bone morphogenic protein co-signaling and regulation of microRNAs. ACS Nano, 8(10), pp. 9941-9953. (doi:10.1021/nn504767g) (PMID:25227207)

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Abstract

It is emerging that nanotopographical information can be used to induce osteogenesis from mesenchymal stromal cells from the bone marrow and it is hoped that this nanoscale bioactivity can be utilized to engineer next generation implants. However, the osteogenic mechanism of surfaces is currently poorly understood. In this report, we investigate mechanism and implicate bone morphogenic protein (BMP) in up-regulation of RUNX2 and show that RUNX2 and its regulatory miRNAs are BMP sensitive. Our data demonstrates that osteogenic nanotopography promotes co-localization of intergrins and BMP2 receptors in order to enhance osteogenic activity and that vitronectin is important in this interface. This provides insight that topographical regulation of adhesion can have effects on signaling cascades outside of cytoskeletal signaling and that adhesions can have roles in augmenting BMP signaling.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and Alakpa, Miss Enateri and Burgess, Dr Karl and McNamara, Dr Laura and Meek, Professor Dominic and Gadegaard, Professor Nikolaj and Yang, Dr Jingli
Authors: Yang, J., McNamara, L. E., Gadegaard, N., Alakpa, E. V., Burgess, K. V., Meek, D., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN:1936-0851
ISSN (Online):1936-086X
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in ACS Nano 8(10):9941-9953
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
438161Stem Cell Differentiation & Genomic Processes in Response to Bioactive NanotopographyMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/G008868/1RI MOLECULAR CELL & SYSTEMS BIOLOGY