Zhou, X., Sun, P., Lucendo-Villarin, B., Angus, A. G.N., Szkolnicka, D., Cameron, K., Farnworth, S. L., Patel, A. H. and Hay, D. C. (2014) Modulating innate immunity improves Hepatitis C virus infection and replication in stem cell-derived hepatocytes. Stem Cell Reports, 3(1), pp. 204-214. (doi: 10.1016/j.stemcr.2014.04.018) (PMID:25068132) (PMCID:PMC4110790)
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Abstract
In this study, human embryonic stem cell-derived hepatocytes (hESC-Heps) were investigated for their ability to support hepatitis C virus (HCV) infection and replication. hESC-Heps were capable of supporting the full viral life cycle, including the release of infectious virions. Although supportive, hESC-Hep viral infection levels were not as great as those observed in Huh7 cells. We reasoned that innate immune responses in hESC-Heps may lead to the low level of infection and replication. Upon further investigation, we identified a strong type III interferon response in hESC-Heps that was triggered by HCV. Interestingly, specific inhibition of the JAK/STAT signaling pathway led to an increase in HCV infection and replication in hESC-Heps. Of note, the interferon response was not evident in Huh7 cells. In summary, we have established a robust cell-based system that allows the in-depth study of virus-host interactions in vitro.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Angus, Dr Allan and Patel, Professor Arvind and Zhou, Dr Xiaoling |
| Authors: | Zhou, X., Sun, P., Lucendo-Villarin, B., Angus, A. G.N., Szkolnicka, D., Cameron, K., Farnworth, S. L., Patel, A. H., and Hay, D. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Stem Cell Reports |
| Publisher: | Elsevier Inc. |
| ISSN: | 2213-6711 |
| ISSN (Online): | 2213-6711 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Stem Cell Reports 3(1):204-214 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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