Virus and host factors affecting the clinical outcome of bluetongue virus infection

Caporale, M., Di Gialleonorado, L., Janowicz, A., Wilkie, G., Shaw, A., Savini, G., Van Rijn, P. A., Mertens, P., Di Ventura, M. and Palmarini, M. (2014) Virus and host factors affecting the clinical outcome of bluetongue virus infection. Journal of Virology, 88(18), pp. 10399-10411. (doi:10.1128/JVI.01641-14) (PMID:24991012) (PMCID:PMC4178883)

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Abstract

Bluetongue is a major infectious disease of ruminants caused by bluetongue virus (BTV), an arbovirus transmitted by Culicoides. Here, we assessed virus and host factors influencing the clinical outcome of BTV infection using a single experimental framework. We investigated how mammalian host species, breed, age, BTV serotypes, and strains within a serotype, affect the clinical course of bluetongue. Results obtained indicate that in small ruminants there is a marked difference in the susceptibility to clinical disease induced by BTV at the host species level, but less so at the breed level. No major differences in virulence were found between divergent serotypes (BTV-8 and BTV-2). However, we observed striking differences in virulence between closely related strains of the same serotype collected towards the beginning and the end of the European BTV-8 outbreak. As observed previously, differences in disease severity were also observed when animals were infected with either blood from a BTV-infected animal or from the same virus isolated in cell culture. Interestingly, with the exception of two silent mutations, full viral genome sequencing showed identical consensus sequences of the virus before and after cell culture isolation. However, deep sequencing analysis revealed a marked decrease in the genetic diversity of the viral population after passaging in mammalian cells. In contrast, passaging in Culicoides cells increased the overall number of low frequency variants compared to virus never passaged in cell culture. Thus, Culicoides might be a source of new viral variants and viral population diversity can be another factor influencing BTV virulence.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Palmarini, Professor Massimo and Wilkie, Dr Gavin and Mertens, Professor Peter Paul Clem and Caporale, Dr Marco and Shaw, Dr Andrew
Authors: Caporale, M., Di Gialleonorado, L., Janowicz, A., Wilkie, G., Shaw, A., Savini, G., Van Rijn, P. A., Mertens, P., Di Ventura, M., and Palmarini, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
Publisher:American Society for Microbiology
ISSN:0022-538X
ISSN (Online):1098-5514
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Journal of Virology 88(18):10399-10411
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
544121Phylogeography, transmission dynamics and pathogenesis of bluetongueMassimo PalmariniWellcome Trust (WELLCOME)092806/Z/10/ZMVLS III - CENTRE FOR VIRUS RESEARCH