Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection

Tran, C. S. et al. (2014) Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection. Cell Host and Microbe, 15(5), pp. 636-643. (doi: 10.1016/j.chom.2014.04.007) (PMID:24832456) (PMCID:PMC4062193)

Full text not currently available from Enlighten.


The mucosal epithelium consists of polarized cells with distinct apical and basolateral membranes that serve as functional and physical barriers to external pathogens. The apical surface of the epithelium constitutes the first point of contact between mucosal pathogens, such as Pseudomonas aeruginosa, and their host. We observed that binding of P. aeruginosa aggregates to the apical surface of polarized cells led to the striking formation of an actin-rich membrane protrusion with inverted polarity, containing basolateral lipids and membrane components. Such protrusions were associated with a spatially localized host immune response to P. aeruginosa aggregates that required bacterial flagella and a type III secretion system apparatus. Host protrusions formed de novo underneath bacterial aggregates and involved the apical recruitment of a Par3/Par6α/aPKC/Rac1 signaling module for a robust, spatially localized host NF-κB response. Our data reveal a role for spatiotemporal epithelial polarity changes in the activation of innate immune responses.

Item Type:Articles
Additional Information:Financial support was provided by EMBO (Y.E.), NIH RO1 AI065902 (J.N.E.), PO1 AI53194 (J.N.E. and K.E.M.), K99CA163535 (D.M.B), K12 HD072222 (C.S.T.), K12 HD000850 (C.S.T.), and K08 DK068358 (P.B.).
Glasgow Author(s) Enlighten ID:Bryant, Dr David
Authors: Tran, C. S., Eran, Y., Ruch, T. R., Bryant, D. M., Datta, A., Brakeman, P., Kierbel, A., Wittmann, T., Metzger, R. J., Mostov, K. E., and Engel, J. N.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Cell Host and Microbe
Publisher:Elsevier (Cell Press)
ISSN (Online):1934-6069

University Staff: Request a correction | Enlighten Editors: Update this record