plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle

Anderson, M., Ng, S.S., Marchesi, V., MacIver, F.H., Stevens, F.E., Riddell, T., Glover, D.M., Hagan, I.M. and McInerny, C.J. (2002) plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. EMBO Journal, 21, pp. 5745-5755. (doi: 10.1093/emboj/cdf564)

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Abstract

The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M–G<sub>1</sub> interval during the fission yeast mitotic cell cycle. A sequence is identified in the <i>cdc15</i><sup>+</sup> promoter that we call a PCB (pombe cell cycle box), which confers M–G<sub>1</sub> -specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, <i>spo12</i><sup>+</sup>, <i>cdc19</i><sup>+</sup>, <i>fin1</i><sup>+</sup>, <i>sid2</i><sup>+</sup>, <i>ppb1</i><sup>+</sup>, <i>mid1</i><sup>+</sup><i>/dmf1</i><sup>+</sup> and <i>plo1</i><sup>+</sup>, which we find to be transcribed at M–G<sub>1</sub>. A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInerny, Dr Chris
Authors: Anderson, M., Ng, S.S., Marchesi, V., MacIver, F.H., Stevens, F.E., Riddell, T., Glover, D.M., Hagan, I.M., and McInerny, C.J.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:EMBO Journal
ISSN:0261-4189
ISSN (Online):1460-2075

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