Nunes, S. F., Hamers, C., Ratinier, M., Shaw, A., Brunet, S., Hudelet, P. and Palmarini, M. (2014) A synthetic biology approach for a vaccine platform against known and newly emerging serotypes of bluetongue virus. Journal of General Virology, 29(7), pp. 912-920. (doi: 10.1128/JVI.02183-14) (PMID:25142610) (PMCID:PMC4248921)
|
Text
96853.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
Bluetongue is one of the major infectious diseases of ruminants and is caused by Bluetongue virus (BTV), an arbovirus existing in nature in at least 26 distinct serotypes. Here, we describe the development of a vaccine platform for BTV. The advent of synthetic biology approaches and the development of reverse genetics systems, has allowed the rapid and reliable design and production of pathogen genomes which can be subsequently manipulated for vaccine production. We describe BTV vaccines based on “synthetic” viruses in which the outer core proteins of different BTV serotypes are incorporated into a common tissue-culture adapted backbone. As a means of validation for this approach, we selected two BTV-8 synthetic reassortants and demonstrated their ability to protect sheep against virulent BTV-8 challenge. In addition, to further highlight the possibilities of genome manipulation for vaccine production, we also designed and rescued a synthetic BTV chimera containing a VP2 protein including regions derived from both BTV-1 and BTV-8. Interestingly, while the parental viruses were neutralized only by homologous antisera, the chimeric proteins could be neutralized by both BTV-1 and BTV-8 antisera. These data suggest that neutralizing epitopes are present in different areas of the BTV VP2 and likely “bivalent” strains eliciting neutralizing antibodies for multiple strains can be obtained.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Ratinier, Dr Maxime and Palmarini, Professor Massimo and Shaw, Dr Andrew |
Authors: | Nunes, S. F., Hamers, C., Ratinier, M., Shaw, A., Brunet, S., Hudelet, P., and Palmarini, M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Journal of General Virology |
Publisher: | Society for General Microbiology |
ISSN: | 0022-1317 |
ISSN (Online): | 1098-5514 |
Copyright Holders: | Copyright © 2014 Nunes et al. |
First Published: | First published in Journal of Virology 29(7):912-920 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record