New applications for phage integrases

Fogg, P. C.M., Colloms, S. , Rosser, S., Stark, M. and Smith, M. C.M. (2014) New applications for phage integrases. Journal of Molecular Biology, 426(15), pp. 2703-2716. (doi:10.1016/j.jmb.2014.05.014) (PMID:24857859) (PMCID:PMC4111918)

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Abstract

Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here, we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimization, biocomputing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rosser, Professor Susan and Stark, Professor William and Colloms, Dr Sean
Authors: Fogg, P. C.M., Colloms, S., Rosser, S., Stark, M., and Smith, M. C.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Molecular Biology
Publisher:Elsevier Ltd.
ISSN:0022-2836
ISSN (Online):1089-8638
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Journal of Molecular Biology 426(15):2703-2716
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
589142A platform for rapid and precise DNA module rearrangements in Synthetic BiologyWilliam StarkBiotechnology and Biological Sciences Research Council (BBSRC)BB/K003356/1RI MOLECULAR CELL & SYSTEMS BIOLOGY