Probing the binding sites of exchanged chlorophyll a in LH2 by Raman and site-selection fluorescence spectroscopies

Gall, A., Robert, B., Cogdell, R. J. , Bellissent-Funel, M.-C. and Fraser, N. J.> (2001) Probing the binding sites of exchanged chlorophyll a in LH2 by Raman and site-selection fluorescence spectroscopies. FEBS Letters, 491(1-2), pp. 143-147. (doi:10.1016/S0014-5793(01)02161-5)

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Publisher's URL: http://dx.doi.org/10.1016/S0014-5793(01)02161-5

Abstract

In this work we have selectively released the 800 nm absorbing bacteriochlorophyll a molecules of the LH2 protein from the photosynthetic bacterium <i>Rhodopseudomonas acidophila</i>, strain 10050, and replaced them with chlorophyll <i>a</i> (Chl<i>a</i>). A combination of low-temperature electronic absorption, resonance Raman and site-selection fluorescence spectroscopies revealed that the Chl<i>a</i> pigments are indeed bound in the B800 binding site; this is the first work that formally proves that such non-native chlorins can be inserted correctly into LH2.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Fraser, Dr Niall and Cogdell, Professor Richard
Authors: Gall, A., Robert, B., Cogdell, R. J., Bellissent-Funel, M.-C., and Fraser, N. J.>
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:FEBS Letters
Publisher:Elsevier BV
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:20 February 2001

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