An arabidopsis stomatin-like protein affects mitochondrial respiratory supercomplex organization

Gehl, B., Lee, C. P., Bota, P., Blatt, M. R. and Sweetlove, L. J. (2014) An arabidopsis stomatin-like protein affects mitochondrial respiratory supercomplex organization. Plant Physiology, 164(3), pp. 1389-1400. (doi:10.1104/pp.113.230383)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1104/pp.113.230383

Abstract

Stomatins belong to the band-7 protein family, a diverse group of conserved eukaryotic and prokaryotic membrane proteins involved in the formation of large protein complexes as protein-lipid scaffolds. The Arabidopsis (Arabidopsis thaliana) genome contains two paralogous genes encoding stomatin-like proteins (SLPs; AtSLP1 and AtSLP2) that are phylogenetically related to human SLP2, a protein involved in mitochondrial fusion and protein complex formation in the mitochondrial inner membrane. We used reverse genetics in combination with biochemical methods to investigate the function of AtSLPs. We demonstrate that both SLPs localize to mitochondrial membranes. SLP1 migrates as a large (approximately 3 MDa) complex in blue-native gel electrophoresis. Remarkably, slp1 knockout mutants have reduced protein and activity levels of complex I and supercomplexes, indicating that SLP affects the assembly and/or stability of these complexes. These findings point to a role for SLP1 in the organization of respiratory supercomplexes in Arabidopsis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sweetlove, Dr Lee and Blatt, Professor Michael and Gehl, Ms Bernadette
Authors: Gehl, B., Lee, C. P., Bota, P., Blatt, M. R., and Sweetlove, L. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Physiology
Publisher:American Society of Plant Biologists
ISSN:0032-0889
ISSN (Online):1532-2548

University Staff: Request a correction | Enlighten Editors: Update this record