Regulation of human genome expression and RNA splicing by human papillomavirus 16 E2 protein

Gauson, E. J., Windle, B., Donaldson, M. , Cafarel, M. M., Dornan, E., Coleman, N., Herzyk, P. , Henderson, S. C., Wang, X. and Morgan, I. (2014) Regulation of human genome expression and RNA splicing by human papillomavirus 16 E2 protein. Virology, 468-70, pp. 10-18. (doi:10.1016/j.virol.2014.07.022)

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Abstract

Human papillomavirus 16 (HPV16) is causative in human cancer. The E2 protein regulates transcription from and replication of the viral genome; the role of E2 in regulating the host genome has been less well studied. We have expressed HPV16 E2 (E2) stably in U2OS cells; these cells tolerate E2 expression well and gene expression analysis identified 74 genes showing differential expression specific to E2. Analysis of published gene expression data sets during cervical cancer progression identified 20 of the genes as being altered in a similar direction as the E2 specific genes. In addition, E2 altered the splicing of many genes implicated in cancer and cell motility. The E2 expressing cells showed no alteration in cell growth but were altered in cell motility, consistent with the E2 induced altered splicing predicted to affect this cellular function. The results present a model system for investigating E2 regulation of the host genome.

Item Type:Articles (Other)
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Morgan, Professor Iain and Herzyk, Dr Pawel and Dornan, Mr Edward and Donaldson, Dr Mary
Authors: Gauson, E. J., Windle, B., Donaldson, M., Cafarel, M. M., Dornan, E., Coleman, N., Herzyk, P., Henderson, S. C., Wang, X., and Morgan, I.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Virology
Publisher:Elsevier Inc.
ISSN:0042-6822
ISSN (Online):1096-0341

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