Proteomics of vitreous humor of patients with exudative age-related macular degeneration

Koss, M. J. et al. (2014) Proteomics of vitreous humor of patients with exudative age-related macular degeneration. PLoS ONE, 9(5), e96895. (doi: 10.1371/journal.pone.0096895) (PMID:24828575) (PMCID:PMC4020801)

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Abstract

Background: There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD).<p></p> Methods and Findings: Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idiopathic floaters) were analyzed with capillary electrophoresis coupled to mass spectrometry in this retrospective case series to define potential candidate protein markers of AMD. Nineteen proteins were found to be upregulated in vitreous of AMD patients. Most of the proteins were plasma derived and involved in biological (ion) transport, acute phase inflammatory reaction, and blood coagulation. A number of proteins have not been previously associated to AMD including alpha-1-antitrypsin, fibrinogen alpha chain and prostaglandin H2-D isomerase. Alpha-1-antitrypsin was validated in vitreous of an independent set of AMD patients using Western blot analysis. Further systems biology analysis of the data indicated that the observed proteomic changes may reflect upregulation of immune response and complement activity.<p></p> Conclusions: Proteome analysis of vitreous samples from patients with AMD, which underwent an intravitreal combination therapy including a core vitrectomy, steroids and bevacizumab, revealed apparent AMD-specific proteomic changes. The identified AMD-associated proteins provide some insight into the pathophysiological changes associated with AMD.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mullen, Dr Bill and Husi, Dr Holger and Mischak, Professor Harald
Authors: Koss, M. J., Hoffmann, J., Nguyen, N., Pfister, M., Mischak, H., Mullen, W., Husi, H., Rejdak, R., Koch, F., Jankowski, J., Krueger, K., Bertelmann, T., Klein, J., Schanstra, J. P., and Siwy, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in PLoS One 9(5):e96895
Publisher Policy:Reproduced under a Creative Commons License

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