A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants

Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B., Clements, G.B. and Thomas, D.G.T. (1987) A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants. Journal of Neuro-Oncology, 4(4), pp. 389-396. (doi: 10.1007/BF00195610)

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Publisher's URL: http://dx.doi.org/10.1007/BF00195610

Abstract

Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV specified proteins, and one monoclonal antibody TI81 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. TI81 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the TI81-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV I infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Peter
Authors: Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B., Clements, G.B., and Thomas, D.G.T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Neuro-Oncology
Publisher:Springer Verlag
ISSN:0167-594X
ISSN (Online):1573-7373

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