Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands

Daly, C. J. , Parmryd, I. and McGrath, J. C. (2012) Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands. Methods in Molecular Biology, 897, pp. 95-107. (doi: 10.1007/978-1-61779-909-9_5)

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Abstract

The use of fluorescent ligands to analyze receptor distribution is increasing in popularity. This is due to the ever growing number of fluorescent ligands and the increased sensitivity of microscope-based technologies. Image-analysis methods have advanced to a stage where quantification of fluorescent signals is relatively simple (if used appropriately). In this chapter we describe a method of analyzing the 2D and 3D distribution of fluorescent ligands in segments of blood vessels. In addition, we introduce the issues surrounding the accurate analysis of colocalization of two different fluorescent ligands.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McGrath, Professor John and Daly, Professor Craig
Authors: Daly, C. J., Parmryd, I., and McGrath, J. C.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Methods in Molecular Biology
Publisher:Humana Press
ISSN:1064-3745

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