Reactivation of latent herpes simplex virus from dissociated identified dorsal root ganglion cells in culture

Kennedy, P. G. E., Al-Saadi, S. A. and Clements, G. B. (1983) Reactivation of latent herpes simplex virus from dissociated identified dorsal root ganglion cells in culture. Journal of General Virology, 64(7), pp. 1629-1635. (doi:10.1099/0022-1317-64-7-1629)

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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-64-7-1629

Abstract

Herpes simplex virus (HSV) types 1 and 2 reactivate from dissociated cultured dorsal root ganglia of latently infected mice. The neurons in culture were identified morphologically and by using the specific anti-neuronal monoclonal antibody A2B5. HSV antigen expression during reactivation was first seen in neurons on day 3 after dissociation, and infectious virus was released subsequently. Approximately 0.4% of neurons released infectious virus. The presence of neutralizing antibody to HSV did not modify the reactivation process. After infection of mouse neuronal cultures in vitro with HSV, cytopathic effect and viral antigen expression appeared within 18 h predominantly in fibroblastic cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Peter
Authors: Kennedy, P. G. E., Al-Saadi, S. A., and Clements, G. B.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

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