Differential susceptibility of human neural cell types in culture to infection with herpes simplex virus

Kennedy, P. G. E., Clement, G. B. and Brown, S. M. (1983) Differential susceptibility of human neural cell types in culture to infection with herpes simplex virus. Brain, 106(1), pp. 101-119. (doi:10.1093/brain/106.1.101)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1093/brain/106.1.101

Abstract

The ability of herpes simplex virus (HSV)-1 Glasgow (strain 17), HSV-2 (strain HG52), temperature sensitive mutants and a thymidine kinase negative mutant of Glasgow strain 17 to produce a cytopathic effect and induce viral antigen expression in primary and passaged human fetal brain cells was studied. No difference was detected between the behaviour of HSV-1 and HSV-2. Cell-type-specific markers and indirect immunofluorescence were used to define unambiguously the type of individual cells in culture. Cells characterized as astrocytes were present in passaged cultures. Neurons were only seen in primary cultures and were markedly nonpermissive for herpes simplex virus as defined by both cytopathic effect and antigen expression when compared with the other cell types. These techniques and observations are seen to have potential significance for a variety of clinical neurological and neurobiological studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Peter
Authors: Kennedy, P. G. E., Clement, G. B., and Brown, S. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Brain
Publisher:Oxford University Press
ISSN:0006-8950
ISSN (Online):1460-2156

University Staff: Request a correction | Enlighten Editors: Update this record