McFarlane, M. et al. (2014) Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. PLoS Neglected Tropical Diseases, 8(7), e2994. (doi: 10.1371/journal.pntd.0002994) (PMID:25058001) (PMCID:PMC4109886)
|
Text
95413.pdf - Published Version Available under License Creative Commons Attribution. 973kB |
Publisher's URL: http://dx.doi.org/10.1371/journal.pntd.0002994
Abstract
Replication of arboviruses in their arthropod vectors is controlled by innate immune responses. The RNA sequence-specific break down mechanism, RNA interference (RNAi), has been shown to be an important innate antiviral response in mosquitoes. In addition, immune signaling pathways have been reported to mediate arbovirus infections in mosquitoes; namely the JAK/STAT, immune deficiency (IMD) and Toll pathways. Very little is known about these pathways in response to chikungunya virus (CHIKV) infection, a mosquito-borne alphavirus (Togaviridae) transmitted by aedine species to humans resulting in a febrile and arthralgic disease. In this study, the contribution of several innate immune responses to control CHIKV replication was investigated. In vitro experiments identified the RNAi pathway as a key antiviral pathway. CHIKV was shown to repress the activity of the Toll signaling pathway in vitro but neither JAK/STAT, IMD nor Toll pathways were found to mediate antiviral activities. In vivo data further confirmed our in vitro identification of the vital role of RNAi in antiviral defence. Taken together these results indicate a complex interaction between CHIKV replication and mosquito innate immune responses and demonstrate similarities as well as differences in the control of alphaviruses and other arboviruses by mosquito immune pathways.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Mcdonald, Dr Melanie and Donald, Dr Claire and Misbah, Miss Suzana and Schnettler, Dr Esther and Rainey, Dr Stephanie and Kohl, Professor Alain |
Authors: | McFarlane, M., Arias-Goeta, C., Martin, E., O'Hara, Z., Lulla, A., Mousson, L., Rainey, S. M., Misbah, S., Schnettler, E., Donald, C., Merits, A., Kohl, A., and Failloux, A.-B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | PLoS Neglected Tropical Diseases |
Publisher: | Public Library of Science |
ISSN: | 1935-2727 |
ISSN (Online): | 1935-2735 |
Copyright Holders: | Copyright © 2014 The Authors |
First Published: | First published in PLoS Neglected Tropical Diseases 8(7):e2994 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record