Hay, R. F., Gibson, G. M. , Lee, M. P., Padgett, M. J. and Phillips, D. B. (2014) Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation. Optics Express, 22(15), pp. 18662-18667. (doi: 10.1364/OE.22.018662)
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Publisher's URL: http://dx.doi.org/10.1364/OE.22.018662
Abstract
High-speed video stereo-microscopy relies on illumination from two distinct angles to create two views of a sample from different directions. The 3D trajectory of a microscopic object can then be reconstructed using parallax to combine 2D measurements of its position in each image. In this work, we evaluate the accuracy of 3D particle tracking using this technique, by extending the number of views from two to four directions. This allows us to record two independent sets of measurements of the 3D coordinates of tracked objects, and comparison of these enables measurement and minimisation of the tracking error in all dimensions. We demonstrate the method by tracking the motion of an optically trapped microsphere of 5 μm in diameter, and find an accuracy of 2–5 nm laterally, and 5–10 nm axially, representing a relative error of less than 2.5% of its range of motion in each dimension.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Gibson, Dr Graham and Padgett, Professor Miles and Phillips, Dr David |
| Authors: | Hay, R. F., Gibson, G. M., Lee, M. P., Padgett, M. J., and Phillips, D. B. |
| College/School: | College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Optics Express |
| Publisher: | Optical Society of America |
| ISSN: | 1094-4087 |
| Copyright Holders: | Copyright © 2014 Optical Society of America |
| First Published: | First published in Optics Express 22(15):18662-18667 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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