Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Hay, R. F., Gibson, G. M. , Lee, M. P., Padgett, M. J. and Phillips, D. B. (2014) Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation. Optics Express, 22(15), pp. 18662-18667. (doi: 10.1364/OE.22.018662)

[img]
Preview
Text
95386.pdf - Published Version
Available under License Creative Commons Attribution.

2MB

Publisher's URL: http://dx.doi.org/10.1364/OE.22.018662

Abstract

High-speed video stereo-microscopy relies on illumination from two distinct angles to create two views of a sample from different directions. The 3D trajectory of a microscopic object can then be reconstructed using parallax to combine 2D measurements of its position in each image. In this work, we evaluate the accuracy of 3D particle tracking using this technique, by extending the number of views from two to four directions. This allows us to record two independent sets of measurements of the 3D coordinates of tracked objects, and comparison of these enables measurement and minimisation of the tracking error in all dimensions. We demonstrate the method by tracking the motion of an optically trapped microsphere of 5 μm in diameter, and find an accuracy of 2–5 nm laterally, and 5–10 nm axially, representing a relative error of less than 2.5% of its range of motion in each dimension.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gibson, Dr Graham and Phillips, Dr David and Padgett, Professor Miles
Authors: Hay, R. F., Gibson, G. M., Lee, M. P., Padgett, M. J., and Phillips, D. B.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
Copyright Holders:Copyright © 2014 Optical Society of America
First Published:First published in Optics Express 22(15):18662-18667
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
542471Optical Atomic Force MicroscopyMiles PadgettEngineering & Physical Sciences Research Council (EPSRC)EP/I007822/1P&A - PHYSICS & ASTRONOMY