Utilising polyphenols for the clinical management of Candida albicans biofilms

Shahzad, M., Sherry, L., Rajendran, R., Edwards, C. A. , Combet, E. and Ramage, G. (2014) Utilising polyphenols for the clinical management of Candida albicans biofilms. International Journal of Antimicrobial Agents, 44(3), pp. 269-273. (doi:10.1016/j.ijantimicag.2014.05.017) (PMID:25104135)

[img]
Preview
Text
95127.pdf - Accepted Version

642kB

Abstract

Polyphenols (PPs) are secondary metabolites abundant in plant-derived foods. They are reported to exhibit antimicrobial activity that may offer an alternative to existing antimicrobials. The aim of this study was to evaluate the antifungal potential of PPs against Candida albicans biofilms that are commonly recalcitrant to antifungal therapy. The antifungal activity of 14 PPs was assessed in terms of planktonic and sessile minimum inhibitory concentrations (PMICs and SMICs, respectively) against various C. albicans clinical isolates. The most active PPs were further tested for their effect on C. albicans adhesion and biofilm growth using standard biomass assays, microscopy and quantitative gene expression. Of the 14 PPs tested, 7 were effective inhibitors of planktonic growth, of which pyrogallol (PYG) was the most effective (PMIC50 = 78 μg/mL), followed by curcumin (CUR) (PMIC50 = 100 μg/mL) and pyrocatechol (PMIC50 = 625 μg/mL). Both PYG and CUR displayed activity against C. albicans biofilms (SMIC50 = 40 μg/mL and 50 μg/mL, respectively), although they did not disrupt the biofilm or directly affect the cellular structure. Overall, CUR displayed superior biofilm activity, significantly inhibiting initial cell adhesion following pre-coating (P < 0.01), biofilm growth (P < 0.05) and gene expression (P < 0.05). This inhibitory effect diminished with prolonged CUR exposure, although it still inhibited by 50% after 4 h adhesion. Overall, CUR exhibited positive antibiofilm properties that could be used at the basis for development of similar molecules, although further cellular and in vivo studies are required to explore its precise mechanism of action.

Item Type:Articles
Additional Information:NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Antimicrobial Agents. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Antimicrobial Agents, [Vol 44(3):269-273 September 2014] DOI: 10.1016/j.ijantimicag.2014.05.017
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shahzad, Dr Muhammad and Ramage, Professor Gordon and Edwards, Professor Christine and Rajendran, Dr Ranjith and Combet Aspray, Dr Emilie and Sherry, Dr Leighann
Authors: Shahzad, M., Sherry, L., Rajendran, R., Edwards, C. A., Combet, E., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:International Journal of Antimicrobial Agents
Publisher:Elsevier B.V.
ISSN:0924-8579
ISSN (Online):1872-7913
Copyright Holders:Copyright © 2014 Elsevier B.V.
First Published:First published in International Journal of Antimicrobial Agents 44(3):269-273
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record