Two-photon imaging

Ricard, C., Coles, J. A., Serduc, R., van der Sanden, B., Verant, P. and Vial, J.-C. (2009) Two-photon imaging. In: Squire, L. R. (ed.) Encyclopedia of Neuroscience. Elsevier: Amsterdam, pp. 1221-1229. ISBN 9780080450469 (doi: 10.1016/B978-008045046-9.00863-9)

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A two-photon microscope is basically a scanning confocal microscope that uses for excitation near-infrared light delivered in intense, femtosecond pulses. It allows imaging with the spatial resolution of a conventional optical microscope (<1 μm) but can perform such imaging through several hundred micrometers of brain tissue. In this article, the physical principles of two-photon imaging are explained, and imaging in the cortex of the living mouse of neurons, glia, and microvasculature is described. Finally, the advantages of two-photon microscopy for studying neuropathologies including tumors are summarized.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Coles, Dr Jonathan
Authors: Ricard, C., Coles, J. A., Serduc, R., van der Sanden, B., Verant, P., and Vial, J.-C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation

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