Wion, D., Christen, T., Barbier, E. L. and Coles, J. A. (2009) PO2 matters in stem cell culture. Cell Stem Cell, 5(3), pp. 242-243. (doi: 10.1016/j.stem.2009.08.009)
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Publisher's URL: http://dx.doi.org/10.1016/j.stem.2009.08.009
Abstract
About a century ago, conditions were worked out for maintaining growing tissue and cells outside the body. From the beginning, care was taken to maintain cultures at a physiological temperature, and to include precise concentrations of salts and other compounds, but the oxygen concentration in the culture medium was simply the result of letting the medium equilibrate with air. This approach was a reasonable first approximation, given that values of partial pressure of oxygen (PO2) in animal tissues were not measured until over a decade later, and all that mattered seemed to be to provide cells with “enough” oxygen.
| Item Type: | Articles (Letter) |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Coles, Dr Jonathan |
| Authors: | Wion, D., Christen, T., Barbier, E. L., and Coles, J. A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Cell Stem Cell |
| Publisher: | Elsevier |
| ISSN: | 1934-5909 |
| ISSN (Online): | 1875-9777 |
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