PO2 matters in stem cell culture

Wion, D., Christen, T., Barbier, E. L. and Coles, J. A. (2009) PO2 matters in stem cell culture. Cell Stem Cell, 5(3), pp. 242-243. (doi: 10.1016/j.stem.2009.08.009)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1016/j.stem.2009.08.009

Abstract

About a century ago, conditions were worked out for maintaining growing tissue and cells outside the body. From the beginning, care was taken to maintain cultures at a physiological temperature, and to include precise concentrations of salts and other compounds, but the oxygen concentration in the culture medium was simply the result of letting the medium equilibrate with air. This approach was a reasonable first approximation, given that values of partial pressure of oxygen (PO2) in animal tissues were not measured until over a decade later, and all that mattered seemed to be to provide cells with “enough” oxygen.

Item Type:Articles (Letter)
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Coles, Dr Jonathan
Authors: Wion, D., Christen, T., Barbier, E. L., and Coles, J. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cell Stem Cell
Publisher:Elsevier
ISSN:1934-5909
ISSN (Online):1875-9777

University Staff: Request a correction | Enlighten Editors: Update this record