Measurement of cytosolic chloride activityby ion-selective microelectrodes

Coles, J. A. (1995) Measurement of cytosolic chloride activityby ion-selective microelectrodes. In: Kraicer, J. and Dixon, S. J. (eds.) Measurement and Manipulation of Intracellular Ions. Series: Methods in Neurosciences (27). Academic Press, pp. 340-360. ISBN 9780121852979 (doi: 10.1016/S1043-9471(06)80021-2)

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Cl– selective microelectrodes give absolute values of aCli (as distinct from qualitative changes) much more directly than do currently available fluorescent indicators. A Cl– microelectrode is a powerful and elegant tool that allows a direct correlation of electrical activity and changes in aCli. In large cells, such as snail neurons, the power can be increased by simultaneous use of an additional electrode to measure a second ion. They should not be put off by excessive concern with junction and tip potentials: all the achievements of intracellular electrophysiology have been made despite them. Poor selectivity has been an occasional inconvenience of Cl– microelectrodes, but improvements have been made and more are to be expected. Obtaining a clean, low-noise recording of aCli in a small cell requires perseverance, but the experimenter has the satisfaction that success is at least partly related to skill.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Coles, Dr Jonathan
Authors: Coles, J. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Publisher:Academic Press

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