Selective downregulation of an inactivating K+ conductance by analogues of cAMP in mouse Schwann cells

Despeyroux, S., Beaudu-Lange, C., Coles, J. A. and Amédée, T. (1997) Selective downregulation of an inactivating K+ conductance by analogues of cAMP in mouse Schwann cells. Journal of Physiology, 499(3), pp. 655-660.

Full text not currently available from Enlighten.

Publisher's URL: http://jp.physoc.org/content/499/Pt_3/655.full.pdf+html

Abstract

oltage-dependent K+ conductances on Schwann cells in organotypic cultures of mouse dorsal root ganglia were classified as inactivating or sustained (responsible for currents IA and IK, respectively). IA is known to be much reduced on Schwann cells that contact neurites. 2. In the absence of neurones, IA and IK were present. IA, but not IK, was markedly reduced (by 80% after 105 h of treatment) by 2 mM 8-(4-chlorophenylthio)-cAMP (cpt-cAMP), a weakly hydrolysable analogue of cAMP. The effect did not appear for at least 2 h and was maximal after about 100 h. 3. The effect of 1 mM cpt-cAMP was abolished in the presence of an inhibitor of protein kinases, N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulphonamide (H-89, 10 microM). 4. Other analogues of cAMP, but not an analogue of cGMP (8-bromo-cGMP), also reduced IA. 5. We conclude that IA, but not IK, can be downregulated by activation of the protein kinase A pathway.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Coles, Dr Jonathan
Authors: Despeyroux, S., Beaudu-Lange, C., Coles, J. A., and Amédée, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Physiology
Publisher:Wiley
ISSN:0022-3751
ISSN (Online):1469-7793

University Staff: Request a correction | Enlighten Editors: Update this record