Southall, T. D., Terhzaz, S. , Cabrero, P. , Chintapalli, V. R., Evans, J. M., Dow, J. A.T. and Davies, S.-A. (2006) Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases. Physiological Genomics, 26(1), pp. 35-45. (doi: 10.1152/physiolgenomics.00038.2006) (PMID:16609144)
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Abstract
Secretory pathway Ca2+/Mn2+-ATPases (SPCAs) are important for maintenance of cellular Ca2+ and Mn2+ homeostasis, and, to date, all SPCAs have been found to localize to the Golgi apparatus. The single Drosophila SPCA gene (SPoCk) was identified by an in silico screen for novel Ca2+-ATPases. It encoded three SPoCk isoforms with novel, distinct subcellular specificities in the endoplasmic reticulum (ER) and peroxisomes in addition to the Golgi. Furthermore, expression of the peroxisome-associated SPoCk isoform was sexually dimorphic. Overexpression of organelle-specific SPoCk isoforms impacted on cytosolic Ca2+ handling in both cultured Drosophila cells and a transporting epithelium, the Drosophila Malpighian (renal) tubule. Specifically, the ER isoform impacted on inositol (1,4,5)-trisphosphate-mediated Ca2+ signaling and the Golgi isoform impacted on diuresis, whereas the peroxisome isoform colocalized with Ca2+ “spherites” and impacted on calcium storage and transport. Interfering RNA directed against the common exons of the three SPoCk isoforms resulted in aberrant Ca2+ signaling and abolished neuropeptide-stimulated diuresis by the tubule. SPoCk thus contributed to both of the contrasting requirements for Ca2+ in transporting epithelia: to transport or store Ca2+ in bulk without compromising its use as a signal.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cabrero, Mr Pablo and Dow, Professor Julian and Terhzaz, Dr Selim and Davies, Professor Shireen and Chintapalli, Dr Venkateswara |
| Authors: | Southall, T. D., Terhzaz, S., Cabrero, P., Chintapalli, V. R., Evans, J. M., Dow, J. A.T., and Davies, S.-A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Physiological Genomics |
| ISSN: | 1094-8341 |
| ISSN (Online): | 1531-2267 |
| Published Online: | 11 April 2006 |
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