Poly(N-acryloylmorpholine): a simple hydrogel system for temporal and spatial control over cell adhesion

Gorman, M., Chim, Y. H., Hart, A., Riehle, M. O. and Urquhart, A. J. (2014) Poly(N-acryloylmorpholine): a simple hydrogel system for temporal and spatial control over cell adhesion. Journal of Biomedical Materials Research Part A, 102(6), pp. 1809-1815. (doi:10.1002/jbm.a.34853)

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Abstract

N-acryloylmorpholine (NAM) was photo-polymerized to produce the homopolymer poly(N-acryloylmorpholine) (PNAM). PNAM behaves as a physical hydrogel in aqueous solvents, doubling its dry weight over a 2 h period before undergoing dissolution following a second order exponential decay profile. In vitro cellular experiments using mouse myoblasts showed that PNAM acts as an effective spatial cell barrier for 38 h, with slow migration of cells into the PNAM area occurring between 45 and 73 h after cell seeding. At 80 h myoblasts fully occupied the area initially blocked by PNAM. Immunofluorescent staining of myoblasts adjacent to PNAM showed normal cytoskeletal structure and well developed focal adhesions indicating limited PNAM toxicity. This study shows that PNAM is an easy to synthesize physical hydrogel that acts as a temporal and spatial barrier to cell adhesion.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hart, Mr Andrew and Riehle, Dr Mathis
Authors: Gorman, M., Chim, Y. H., Hart, A., Riehle, M. O., and Urquhart, A. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Biomedical Materials Research Part A
Publisher:John Wiley & Sons, Ltd.
ISSN:1549-3296
ISSN (Online):1552-4965

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470563DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING