Alexeev, D. et al. (2003) Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core. ChemBioChem, 4(9), pp. 894-896. (doi: 10.1002/cbic.200300699)
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Publisher's URL: http://www.chembiochem.com
Abstract
A fluoroleucine mutant of ubiquitin (cyan) with two strategically placed fluorine reporter groups in its core was synthesised. 1H NMR, crystallography, CD spectroscopy, biological assay and calorimetry indicate minimal structural and functional perturbation compared to the native protein (yellow; see picture). 19F NMR spectra indicate changes within the core during folding and unfolding.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cooper, Professor Alan and Jamieson, Dr Craig and Price, Prof Nicholas and Kelly, Dr Sharon |
| Authors: | Alexeev, D., Barlow, P.N., Bury, S.M., Charrier, J.D., Cooper, A., Hadfield, D., Jamieson, C., Kelly, S.M., Layfield, R., Mayer, R.J., McSparron, H., Price, N.C., Ramage, R., Sawyer, L., Starkmann, B.A., Uhrin, D., Wilken, J., and Young, D.W. |
| College/School: | College of Medical Veterinary and Life Sciences |
| Journal Name: | ChemBioChem |
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA |
| ISSN: | 1439-4227 |
| ISSN (Online): | 1439-7633 |
| Published Online: | 04 September 2003 |
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