Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core

Alexeev, D. et al. (2003) Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core. ChemBioChem, 4(9), pp. 894-896. (doi:10.1002/cbic.200300699)

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Publisher's URL: http://www.chembiochem.com

Abstract

A fluoroleucine mutant of ubiquitin (cyan) with two strategically placed fluorine reporter groups in its core was synthesised. 1H NMR, crystallography, CD spectroscopy, biological assay and calorimetry indicate minimal structural and functional perturbation compared to the native protein (yellow; see picture). 19F NMR spectra indicate changes within the core during folding and unfolding.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Alan and Jamieson, Dr Craig and Price, Prof Nicholas and Kelly, Dr Sharon
Authors: Alexeev, D., Barlow, P.N., Bury, S.M., Charrier, J.D., Cooper, A., Hadfield, D., Jamieson, C., Kelly, S.M., Layfield, R., Mayer, R.J., McSparron, H., Price, N.C., Ramage, R., Sawyer, L., Starkmann, B.A., Uhrin, D., Wilken, J., and Young, D.W.
College/School:College of Medical Veterinary and Life Sciences
Journal Name:ChemBioChem
Publisher:Wiley - V C H Verlag GmbH & Co. KGaA
ISSN:1439-4227
ISSN (Online):1439-7633
Published Online:04 September 2003

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