Gentamicin-attenuated leishmania infantum vaccine: protection of dogs against canine visceral leishmaniosis in endemic area of southeast of Iran

Daneshvar, H., Namazi, M. J., Kamiabi, H., Burchmore, R. , Cleaveland, S. and Phillips, S. (2014) Gentamicin-attenuated leishmania infantum vaccine: protection of dogs against canine visceral leishmaniosis in endemic area of southeast of Iran. PLoS Neglected Tropical Diseases, 8(4), e2757. (doi:10.1371/journal.pntd.0002757)

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Publisher's URL: http://dx.doi.org/10.1371/journal.pntd.0002757

Abstract

An attenuated line of Leishmania infantum (L. infantum H-line) has been established by culturing promastigotes in vitro under gentamicin pressure. A vaccine trial was conducted using 103 naive dogs from a leishmaniosis non-endemic area (55 vaccinated and 48 unvaccinated) brought into an endemic area of southeast Iran. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination. The efficacy of the vaccine was evaluated after 24 months (4 sandfly transmission seasons) by serological, parasitological analyses and clinical examination. In western blot analysis of antibodies to L. infantum antigens, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs which were seropositive at >100, recognized the 21 kDa antigen of L. infantum wild-type (WT). Nine out of 31 (29%) unvaccinated dogs, but none of vaccinated dogs, were positive for the presence of Leishmania DNA. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. These results suggest that gentamicin-attenuated L. infantum induced a significant and strong protective effect against canine visceral leishmaniosis in the endemic area.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Namazi, Dr Mohammad and Cleaveland, Professor Sarah and Daneshvar, Dr Hamid and Phillips, Professor Stephen
Authors: Daneshvar, H., Namazi, M. J., Kamiabi, H., Burchmore, R., Cleaveland, S., and Phillips, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS Neglected Tropical Diseases
Publisher:Public Library of Science
ISSN:1935-2727
ISSN (Online):1935-2735
Copyright Holders:Copyright © 2014 The Authors
First Published:First published inPLoS Neglected Tropical Diseases 8(4):e2757
Publisher Policy:Reproduced under a Creative Commons License

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