Dynamics of bovine intramammary infections due to coagulase-negative staphylococci on four farms

Bexiga, R., Rato, M. G., Lemsaddek, A., Semedo-Lemsaddek, T., Carneiro, C., Pereira, H., Mellor, D. J. , Ellis, K. A. and Vilela, C. L. (2014) Dynamics of bovine intramammary infections due to coagulase-negative staphylococci on four farms. Journal of Dairy Research, 81(02), pp. 208-214. (doi:10.1017/S0022029914000041) (PMID:24594229)

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Publisher's URL: http://dx.doi.org/10.1017/S0022029914000041

Abstract

The objectives of this study were to compare the impact of different coagulase-negative species (CNS) on udder health measured in terms of individual quarter milk somatic cell count (SCC) and duration of intramammary infection, and to get some insight into most likely routes of infection for different CNS species. This longitudinal observational study was performed on four farms that were sampled at 4-week intervals for a total of 12 visits each. Quarters infected with CNS were followed through time with milk samples being submitted for bacteriological culture and SCC determination. PCR amplification of the internal transcribed spacer region and sequencing of the sodA and rpoB genes were used for species allocation. Pulsed-field gel electrophoresis (PFGE) was performed to assess strain identity. The percentage of quarters affected per farm varied between 6 and 35%, with the most frequently isolated CNS species being Staphylococcus epidermidis, followed by Staph. simulans, Staph. chromogenes and Staph. haemolyticus. It was possible to follow 111 intramammary infections due to CNS through time. Duration of infection had a mean of 188 d and was not significantly different between CNS species. Geometric mean quarter SCC overall was 132 000 cells/ml and was also not significantly different between CNS species. Despite the possibility of a different epidemiology of infection, the impact in terms of udder health seems to be similar for different CNS species.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ellis, Dr Kathryn and Mellor, Professor Dominic
Authors: Bexiga, R., Rato, M. G., Lemsaddek, A., Semedo-Lemsaddek, T., Carneiro, C., Pereira, H., Mellor, D. J., Ellis, K. A., and Vilela, C. L.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Dairy Research
Publisher:Cambridge University Press
ISSN:0022-0299
ISSN (Online):1469-7629
Copyright Holders:Copyright © 2014 Proprietors of Journal of Dairy Research
First Published:First published in Journal of Dairy Research 81(02):208-214
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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