Cell interactions at the nanoscale: piezoelectric stimulation

Curtis, A. S.G., Reid, S., Martin, I. , Vaidyanathan, R., Smith, C.-A. , Nikukar, H. and Dalby, M. J. (2013) Cell interactions at the nanoscale: piezoelectric stimulation. IEEE Transactions on NanoBioscience, 12(3), pp. 247-254. (doi:10.1109/TNB.2013.2257837)

Curtis, A. S.G., Reid, S., Martin, I. , Vaidyanathan, R., Smith, C.-A. , Nikukar, H. and Dalby, M. J. (2013) Cell interactions at the nanoscale: piezoelectric stimulation. IEEE Transactions on NanoBioscience, 12(3), pp. 247-254. (doi:10.1109/TNB.2013.2257837)

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Abstract

Nanometric movements of the substrate on which endothelial cells are growing, driven by periodic sinusoidal vibration from 1 Hz to 50 Hz applied by piezo actuators, upregulate endothelin-1 and Kruppel-like factor 2 expression, and increase cell adhesion. These movements are in the z (vertical) axis and ranges from 5 to 50 nm and are similar in vertical extent to protrusions from the cells themselves already reported in the literature. White noise vibrations do not to produce these effects. Vibrational sweeps, if suitably confined within a narrow frequency range, produce similar stimulatory effects but not at wider sweeps. These effects suggest that coherent vibration is crucial for driving these cellular responses. In addition to this, the applied stimulations are observed to be close to or below the random seismic noise of the surroundings, which may suggest stochastic resonance is being employed. The stimulations also interact with the effects of nanometric patterning of the substrates on cell adhesion and Kruppel-like factor 2 and endothelin-1 expression thus linking cell reactions to nanotopographically patterned surfaces with those to mechanical stimulation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Martin, Dr Iain and Curtis, Professor Adam and Dalby, Professor Matthew and Smith, Mrs Carol-Anne and Reid, Professor Stuart
Authors: Curtis, A. S.G., Reid, S., Martin, I., Vaidyanathan, R., Smith, C.-A., Nikukar, H., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Physics and Astronomy
Journal Name:IEEE Transactions on NanoBioscience
Publisher:IEEE
ISSN:1536-1241
ISSN (Online):1558-2639
Copyright Holders:Copyright © 2013 IEEE
First Published:First published in IEEE Transactions on NanoBioscience 12(3):247-254
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
459311Investigations in Gravitational Radiation.Sheila RowanScience & Technologies Facilities Council (STFC)PP/F001118/1P&A - PHYSICS & ASTRONOMY
459313Investigations in Gravitational Radiation.Sheila RowanScience & Technologies Facilities Council (STFC)ST/J000361/1P&A - PHYSICS & ASTRONOMY