Analysis of the root system architecture of Arabidopsis provides a quantitative readout of crosstalk between nutritional signals

Kellermeier, F., Armengaud, P., Seditas, T. J., Danku, J., Salt, D. E. and Amtmann, A. (2014) Analysis of the root system architecture of Arabidopsis provides a quantitative readout of crosstalk between nutritional signals. Plant Cell, 26(4), pp. 1480-1490. (doi: 10.1105/tpc.113.122101)

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Abstract

As plant roots forage the soil for food and water, they translate a multifactorial input of environmental stimuli into a multifactorial developmental output that manifests itself as root system architecture (RSA). Our current understanding of the underlying regulatory network is limited because root responses have traditionally been studied separately for individual nutrient deficiencies. In this study, we quantified 13 RSA parameters of Arabidopsis thaliana in 32 binary combinations of N, P, K, S, and light. Analysis of variance showed that each RSA parameter was determined by a typical pattern of environmental signals and their interactions. P caused the most important single-nutrient effects, while N-effects were strongly light dependent. Effects of K and S occurred mostly through nutrient interactions in paired or multiple combinations. Several RSA parameters were selected for further analysis through mutant phenotyping, which revealed combinations of transporters, receptors, and kinases acting as signaling modules in K–N interactions. Furthermore, nutrient response profiles of individual RSA features across NPK combinations could be assigned to transcriptionally coregulated clusters of nutrient-responsive genes in the roots and to ionome patterns in the shoots. The obtained data set provides a quantitative basis for understanding how plants integrate multiple nutritional stimuli into complex developmental programs.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Amtmann, Professor Anna and Kellermeier, Mr Fabian
Authors: Kellermeier, F., Armengaud, P., Seditas, T. J., Danku, J., Salt, D. E., and Amtmann, A.
Subjects:Q Science > QK Botany
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Life Sciences
Research Group:Plant Science Group
Journal Name:Plant Cell
Publisher:American Society of Plant Biologists
ISSN:1040-4651
ISSN (Online):1532-298X
Copyright Holders:Copyright © 2014 American Society of Plant Biologists
First Published:First published in Plant Cell 26(4):1480-1490
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
526861Environmental Genetics of Root System ArchitectureAnna AmtmannGatsby Charitable Foundation (GATSBY-CF)GAT2910RI MOLECULAR CELL & SYSTEMS BIOLOGY