Cao, Z., Subramaniam, S. and Bulleid, N. J. (2014) Lack of an efficient endoplasmic reticulum-localized recycling system protects peroxiredoxin IV from hyperoxidation. Journal of Biological Chemistry, 289(9), pp. 5490-5498. (doi: 10.1074/jbc.M113.529305)
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Publisher's URL: http://dx.doi.org/10.1074/jbc.M113.529305
Abstract
Typical 2-cys peroxiredoxins are required to remove hydrogen peroxide from several different cellular compartments. Their activity can be regulated by hyperoxidation and consequent inactivation of the active site peroxidatic cysteine. Here we have developed a simple assay to quantify the hyperoxidation of peroxiredoxins. Hyperoxidation of peroxiredoxins can only occur efficiently in the presence of a recycling system usually based on thioredoxin and thioredoxin reductase. We demonstrate that there is a marked difference in the sensitivity of the endoplasmic reticulum-localized peroxiredoxin to hyperoxidation compared to either the cytosolic or mitochondrial enzymes. Each enzyme is equally sensitive to hyperoxidation in the presence of a robust recycling system. Our results demonstrate that the peroxiredoxin IV recycling in the ER is much less efficient than in the cytosol or mitochondria leading to the protection of peroxiredoxin IV from hyperoxidation.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Cao, Dr Zhenbo and Bulleid, Professor Neil |
Authors: | Cao, Z., Subramaniam, S., and Bulleid, N. J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Journal of Biological Chemistry |
Journal Abbr.: | J Biol Chem. |
Publisher: | American Society for Biochemistry and Molecular Biology, Inc. |
ISSN: | 0021-9258 |
ISSN (Online): | 1083-351X |
Copyright Holders: | Copyright © 2014 The American Society for Biochemistry and Molecular Biology, Inc. |
First Published: | First published in Journal of Biological Chemistry 289(9):5490-5498 |
Publisher Policy: | Reproduced under a Creative Commons License |
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