Resonance assignment of As-p18, a fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum

Ibáñez-Shimabukuro, M., Rey-Burusco, M.F., Cooper, A., Kennedy, M. W., Córsico, B. and Smith, B. O. (2014) Resonance assignment of As-p18, a fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum. Biomolecular NMR Assignments, 8(1), pp. 33-36. (doi:10.1007/s12104-012-9447-1)

[img]
Preview
Text
93227_coversheet.pdf

79kB
[img]
Preview
Text
93227.pdf - Published Version
Available under License Creative Commons Attribution.

762kB

Abstract

As-p18 is produced and secreted by larvae of the parasitic nematode Ascaris suum as they develop within their eggs. The protein is a member of the fatty acid binding protein (FABP) family found in a wide range of eukaryotes, but is distinctive in that it is secreted from the synthesizing cell and has predicted additional structural features not previously seen in other FABPs. As-p18 and similar proteins found only in nematodes have therefore been designated ‘nemFABPs’. Sequence-specific 1H, 13C and 15N resonance assignments were established for the 155 amino acid recombinant protein (18.3 kDa) in complex with oleic acid, using a series of three-dimensional triple-resonance heteronuclear NMR experiments. The secondary structure of As-p18 is predicted to be very similar to other FABPs, but the protein has extended loops that have not been observed in other FABPs whose structures have so far been solved.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Malcolm and Smith, Dr Brian and Cooper, Professor Alan
Authors: Ibáñez-Shimabukuro, M., Rey-Burusco, M.F., Cooper, A., Kennedy, M. W., Córsico, B., and Smith, B. O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Chemistry
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Biomolecular NMR Assignments
Publisher:Springer Netherlands
ISSN:1874-2718
ISSN (Online):1874-270X
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in Biomolecular NMR Assignments 8(1):33-36
Publisher Policy:Reproduced under a Creative Commons License
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
465741Structural and biophysical analysis of novel lipid binding proteins from parasitic helminthsMalcolm KennedyWellcome Trust (WELLCOME)083625/Z/07/ZLS - ANIMAL BIOLOGY