Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides

Roszak, A.W., Gardiner, A.T., Isaacs, N.W. and Cogdell, R.J. (2007) Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides. Biochemistry, 46(11), pp. 2909-2916. (doi:10.1021/bi062154i)

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Abstract

This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard and Isaacs, Professor Neil and Roszak, Dr Aleksander
Authors: Roszak, A.W., Gardiner, A.T., Isaacs, N.W., and Cogdell, R.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Chemistry
Journal Name:Biochemistry
ISSN:0006-2979
ISSN (Online):1608-3040
Published Online:23 February 2007

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