Pol5p, a novel binding partner to Cdc10p in fission yeast involved in rRNA production

Nadeem, F.K., Blair, D. and McInerny, C.J. (2006) Pol5p, a novel binding partner to Cdc10p in fission yeast involved in rRNA production. Molecular Genetics and Genomics, 276(4), pp. 391-401. (doi: 10.1007/s00438-006-0144-6)

Abstract

Cdc10p is a major component of the cell cycle transcription factor complex MBF that controls G1-S phase specific gene expression in the fission yeast Schizosaccharomyces pombe. Here, we describe the identification of a new binding partner to Cdc10p and Pol5p. Pol5p was discovered through a 2-hybrid screen, with the direct interaction confirmed by in vitro "pull-down" experiments with bacterially expressed proteins. Pol5p appears to have no role in cell cycle gene expression, but is instead required for rRNA production. Pol5p is an essential gene, expressed constitutively throughout both the mitotic and meiotic life cycles, and localises to the nucleus. Over-expressing Pol5p has no phenotype, but reducing levels of Pol5p inhibits rRNA production. Pol5p is shown to bind to rDNA promoter fragments. Potentially, we have identified a mechanism by which Cdc10p controls rDNA gene expression, therefore linking the cell cycle with cellular growth.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McInerny, Dr Chris
• Authors: Nadeem, F.K., Blair, D., and McInerny, C.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Molecular Genetics and Genomics
• Journal Abbr.: Mol. Genet. Genomics
• ISSN: 1617-4615
• ISSN (Online): 1617-4623
• Published Online: 01 July 2006