Jones, S., Asokanathan, C., Kmiec, D., Irvine, J., Fleck, R., Xing, D., Moore, B., Parton, R. and Coote, J. (2014) Protein coated microcrystals formulated with model antigens and modified with calcium phosphate exhibit enhanced phagocytosis and immunogenicity. Vaccine, 32(33), pp. 4234-4242. (doi: 10.1016/j.vaccine.2013.09.061)
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Publisher's URL: http://dx.doi.org/10.1016/j.vaccine.2013.09.061
Abstract
Protein-coated microcrystals (PCMCs) were investigated as potential vaccine formulations for a range of model antigens. Presentation of antigens as PCMCs increased the antigen-specific IgG responses for all antigens tested, compared to soluble antigens. When compared to conventional aluminium-adjuvanted formulations, PCMCs modified with calcium phosphate (CaP) showed enhanced antigen-specific IgG responses and a decreased antigen-specific IgG1:IgG2a ratio, indicating the induction of a more balanced Th1/Th2 response. The rate of antigen release from CaP PCMCs, <i>in vitro</i>, decreased strongly with increasing CaP loading but their immunogenicity in vivo was not significantly different, suggesting the adjuvanticity was not due to a depot effect. Notably, it was found that CaP modification enhanced the phagocytosis of fluorescent antigen-PCMC particles by J774.2 murine monocyte/macrophage cells compared to soluble antigen or soluble PCMCs. Thus, CaP PCMCs may provide an alternative to conventional aluminium-based acellular vaccines to provide a more balanced Th1/Th2 immune response.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Xing, Dr Dorothy and Coote, Professor John and Parton, Dr Roger and Irvine, Ms June and Jones, Dr Sarah |
| Authors: | Jones, S., Asokanathan, C., Kmiec, D., Irvine, J., Fleck, R., Xing, D., Moore, B., Parton, R., and Coote, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Vaccine |
| Publisher: | Elsevier |
| ISSN: | 0264-410X |
| ISSN (Online): | 1873-2518 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Vaccine |
| Publisher Policy: | Reproduced under a Creative Commons License |
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