Arachidonic acid metabolites and enzyme transcripts in asthma are altered by cigarette smoking

Thomson, N.C. et al. (2014) Arachidonic acid metabolites and enzyme transcripts in asthma are altered by cigarette smoking. Allergy, 69(4), pp. 527-536. (doi: 10.1111/all.12376)

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Background: Arachidonic acid metabolites are implicated in the pathogenesis of asthma although only limited information is available on the impact of current smoking history on these metabolites. The aim of the study was to examine the effect of smoking status on urinary, sputum, and plasma eicosanoid concentrations and relevant enzyme transcripts in asthma.<p></p> Methods: In 108 smokers and never smokers with asthma and 45 healthy controls [smokers and never smokers], we measured urinary tetranor prostaglandin (PG)D2 (PGDM) and leukotriene (LT)E4, induced sputum fluid LTB4, LTE4, PGD2, and PGE2, plasma secretory phospholipase A2 (sPLA2), and 11β prostaglandin F2α (11βPGF2α), and, in a subgroup with severe asthma, airway leukocyte and epithelial cell mRNA expression levels of arachidonic acid metabolic enzymes.<p></p> Results: Smokers with asthma had higher urinary LTE4; 83 (59, 130) vs 59 (40, 90) pg/mg creatinine, P = 0.008, and PGDM; 60 (35, 100) vs 41 (28, 59) ng/mg creatinine, P = 0.012 concentrations, respectively, and lower sputum PGE2 concentrations 80 (46, 157) vs 192 (91, 301) pg/ml, P = 0.001 than never smokers with asthma. Sputum LTB4(P = 0.013), and plasma 11βPGF2α(P = 0.032), concentrations, respectively, were increased in smokers with asthma compared with healthy smokers. Asthma-specific and smoking-related increases (>1.5-fold expression) in arachidonate 15-lipoxygenase and gamma-glutamyltransferase transcripts were demonstrated.<p></p>

Item Type:Articles
Glasgow Author(s) Enlighten ID:Messow, Dr Martina and Thomson, Professor Neil and Hilmi, Mr Omar and Spears, Dr Mark and Chaudhuri, Dr Rekha and McSharry, Dr Charles and Shepherd, Professor Malcolm
Authors: Thomson, N.C., Chaudhuri, R., Spears, M., Messow, C., Jelinsky, S., Miele, G., Nocka, K., Takahashi, E., Hilmi, O.J., Shepherd, M.C., Miller, D. K., and McSharry, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Health and Wellbeing > Robertson Centre
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Allergy
Publisher:John Wiley & Sons Ltd.
ISSN (Online):1398-9995

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