Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics

Bourquin, Y., Syed, A., Reboud, J., Ranford-Cartwright, L. C., Barrett, M. P. and Cooper, J. M. (2014) Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics. Angewandte Chemie (International Edition), 53(22), pp. 5587-5590. (doi:10.1002/anie.201310401) (PMID:24677583) (PMCID:PMC4232275)

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Abstract

One significant challenge in medical diagnostics lies in the development of label-free methods to separate different cells within complex biological samples. Here we demonstrate a generic, low-power ultrasonic separation technique, able to enrich different cell types based upon their physical properties. For malaria, we differentiate between infected and non-infected red blood cells in a fingerprick-sized drop of blood. We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005 %). In a second example, we also show that our methods can be used to enrich different cell types, concentrating Trypanosoma in blood at very low levels of infection, on disposable, low-cost chips.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ranford-Cartwright, Dr Lisa and Cooper, Professor Jonathan and Reboud, Dr Julien and Syed, Miss Abeer and Barrett, Professor Michael
Authors: Bourquin, Y., Syed, A., Reboud, J., Ranford-Cartwright, L. C., Barrett, M. P., and Cooper, J. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Angewandte Chemie (International Edition)
Publisher:Wiley - V C H Verlag GmbH & Co. KGaA
ISSN:1433-7851
ISSN (Online):1521-3773
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Angewandte Chemie 53(22):5587–5590
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
465001Listening to the Microworld.Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F040857/1ENG - BIOMEDICAL ENGINEERING
371796The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/Z/08/ZIII - PARASITOLOGY
371798The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/B/08/ZIII - PARASITOLOGY