Systems analysis of guard cell membrane transport for enhanced stomatal dynamics and water use efficiency

Wang, Y., Hills, A. and Blatt, M. (2014) Systems analysis of guard cell membrane transport for enhanced stomatal dynamics and water use efficiency. Plant Physiology, 164(4), pp. 1593-1599. (doi:10.1104/pp.113.233403) (PMID:24596330)

[img]
Preview
Text
92877.pdf - Published Version

857kB

Abstract

Stomatal transpiration is at the centre of a crisis in water availability and crop production that is expected to unfold over the next 20-30 years. Global water usage has increased 6-fold in the past 100 years, twice as fast as the human population, and is expected to double again before 2030, driven mainly by irrigation and agriculture. Guard cell membrane transport is integral to controlling stomatal aperture and offers important targets for genetic manipulation to improve crop performance. However, its complexity presents a formidable barrier to exploring such possibilities. With few exceptions, mutations that increase water use efficiency commonly have been found to do so with substantial costs to the rate of carbon assimilation, reflecting the trade-off in CO2 availability with suppressed stomatal transpiration. One approach yet to be explored in any detail relies on quantitative systems analysis of the guard cell. Our deep knowledge of transport and homeostasis in these cells gives real substance to the prospect for ‘reverse engineering’ of stomatal responses, using in silico design in directing genetic manipulation for improved water use and crop yields. Here we address this problem with a focus on stomatal kinetics, taking advantage of the OnGuard software and models of the stomatal guard cell (www.psrg.org.uk) recently developed for exploring stomatal physiology. Our analysis suggests that manipulations of single transporter populations are likely to have unforeseen consequences. Channel gating, especially of the dominant K+ channels, appears the most favorable target for experimental manipulation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hills, Mr Adrian and Blatt, Professor Michael and Wang, Dr Yizhou
Authors: Wang, Y., Hills, A., and Blatt, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Physiology
Publisher:American Society for Plant Biologists
ISSN:0032-0889
ISSN (Online):1532-2548
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Plant Physiology 164(4):1593-1599
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
537651Regulation of membrane fusion by a novel Sec1/Munc18-associated proteinMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/H024867/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
452562Analysis of membrane traffic in adaptive stress tolerance in plantsMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/F001630/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
626121Stomatal-based systems analysis of water use efficiencyMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L001276/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
522471A protein scaffold common to root K+ transport and stomatal water use efficiencyMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/H009817/1RI MOLECULAR CELL & SYSTEMS BIOLOGY