Witte, C., Kremer, C., Chanasakulniyom, M., Reboud, J. , Wilson, R. , Cooper, J. M. and Neale, S. L. (2014) Spatially selecting single cell for lysis using light induced electric fields. Small, 10(15), pp. 3026-3031. (doi: 10.1002/smll.201400247)
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Publisher's URL: http://dx.doi.org/10.1002/smll.201400247
Abstract
An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Neale, Dr Steven and Wilson, Dr Robert and Chanasakulniyom, Miss Mayuree and Witte, Dr Christian and Cooper, Professor Jonathan and Reboud, Dr Julien |
| Authors: | Witte, C., Kremer, C., Chanasakulniyom, M., Reboud, J., Wilson, R., Cooper, J. M., and Neale, S. L. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Small |
| Publisher: | Wiley-VCH Verlag |
| ISSN: | 1613-6810 |
| ISSN (Online): | 1613-6829 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Small 10(15):3026-3031 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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