Spatially selecting single cell for lysis using light induced electric fields

Witte, C., Kremer, C., Chanasakulniyom, M., Reboud, J. , Wilson, R. , Cooper, J. M. and Neale, S. L. (2014) Spatially selecting single cell for lysis using light induced electric fields. Small, 10(15), pp. 3026-3031. (doi:10.1002/smll.201400247)

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Publisher's URL: http://dx.doi.org/10.1002/smll.201400247

Abstract

An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Dr Robert and Chanasakulniyom, Miss Mayuree and Cooper, Professor Jonathan and Reboud, Dr Julien and Witte, Dr Christian and Neale, Dr Steven
Authors: Witte, C., Kremer, C., Chanasakulniyom, M., Reboud, J., Wilson, R., Cooper, J. M., and Neale, S. L.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Small
Publisher:Wiley-VCH Verlag
ISSN:1613-6810
ISSN (Online):1613-6829
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Small 10(15):3026-3031
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
506171Micro-Actuators Controlled by Optoelectronic Tweezers (MACOET)Jonathan CooperThe Royal Academy of Engineering (RAE)EP/G058393/1ENG - BIOMEDICAL ENGINEERING
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING
553521Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and WellbeingJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/I017887/1ENG - BIOMEDICAL ENGINEERING