Decline in presumptively protective gut bacterial species and metabolites are paradoxically associated with disease improvement in pediatric crohn’s disease during enteral nutrition

Gerasimidis, K. et al. (2014) Decline in presumptively protective gut bacterial species and metabolites are paradoxically associated with disease improvement in pediatric crohn’s disease during enteral nutrition. Inflammatory Bowel Diseases, (doi:10.1097/MIB.0000000000000023)

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Abstract

Background: The gut microbiota is implicated in the pathogenesis of Crohn's disease (CD). Exclusive enteral nutrition (EEN) is a successful treatment, but its mode of action remains unknown. This study assessed serial changes in the fecal microbiota milieu during EEN.<p></p> Methods: Five fecal samples were collected from CD children: 4 during EEN (start, 15, 30, end EEN approximately 60 days) and the fifth on habitual diet. Two samples were collected from healthy control subjects. Fecal pH, bacterial metabolites, global microbial diversity abundance, composition stability, and quantitative changes of total and 7 major bacterial groups previously implicated in CD were measured.<p></p> Results: Overall, 68 samples were from 15 CD children and 40 from 21 control subjects. Fecal pH and total sulfide increased and butyric acid decreased during EEN (all P < 0.05). Global bacterial diversity abundance decreased (P <0.05); a higher degree of microbiota composition stability was seen in control subjects than in CD children during EEN (at P <= 0.008). Faecalibacterium prausnitzii spp concentration significantly decreased after 30 days on EEN (P < 0.05). In patients who responded to EEN, the magnitude of the observed changes was greater and the concentration of Bacteroides/Prevotella group decreased (P < 0.05). All these changes reverted to pretreatment levels on free diet, and EEN microbiota diversity increased when the children returned to their free diet.<p></p> Conclusions: EEN impacts on gut microbiota composition and changes fecal metabolic activity. It is difficult to infer a causative association between such changes and disease improvement, but the results do challenge the current perception of a protective role for F. prausnitzii in CD.<p></p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Russell, Dr Richard and Edwards, Professor Christine and Hanske, Dr Laura and Bertz, Mr Martin and Gerasimidis, Dr Konstantinos and McGrogan, Dr Paraic
Authors: Gerasimidis, K., Bertz, M., Hanske, L., Junick, J., Biskou, O., Aguilera, M., Garrick, V., Russell, R. K., Blaut, M., McGrogan, P., and Edwards, C. A.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Inflammatory Bowel Diseases
ISSN:1078-0998
ISSN (Online):1536-4844

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