GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless

Dornan, A.J. , Gailey, D.A. and Goodwin, S.F. (2005) GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless. Genesis, 42, pp. 236-246. (doi: 10.1002/gene.20143) (PMID:16028231)

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Abstract

The fru4 allele of the sex determination gene fruitless is induced by insertion of a P[lacZ,ry+] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male-specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, P[GAL4,w+], recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru-specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3- and P4-derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru-expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Goodwin, Dr Stephen and Dornan, Dr Anthony
Authors: Dornan, A.J., Gailey, D.A., and Goodwin, S.F.
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Genesis
Publisher:Wiley
ISSN:1526-954X
ISSN (Online):1526-968X

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