Donoghue, P. S., Sun, T., Gadegaard, N. , Riehle, M. O. and Barnett, S. C. (2014) Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair. Molecular Pharmaceutics, 11(7), pp. 2143-2150. (doi: 10.1021/mp400526n) (PMID:24279373) (PMCID:PMC4087043)
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Abstract
Tubular scaffolds which incorporate a variety of micro- and nanotopographies have a wide application potential in tissue engineering especially for the repair of spinal cord injury (SCI). We aim to produce metabolically active differentiated tissues within such tubes, as it is crucially important to evaluate the biological performance of the three-dimensional (3D) scaffold and optimize the bioprocesses for tissue culture. Because of the complex 3D configuration and the presence of various topographies, it is rarely possible to observe and analyze cells within such scaffolds in situ. Thus, we aim to develop scaled down mini-chambers as simplified in vitro simulation systems, to bridge the gap between two-dimensional (2D) cell cultures on structured substrates and three-dimensional (3D) tissue culture. The mini-chambers were manipulated to systematically simulate and evaluate the influences of gravity, topography, fluid flow, and scaffold dimension on three exemplary cell models that play a role in CNS repair (i.e., cortical astrocytes, fibroblasts, and myelinating cultures) within a tubular scaffold created by rolling up a microstructured membrane. Since we use CNS myelinating cultures, we can confirm that the scaffold does not affect neural cell differentiation. It was found that heterogeneous cell distribution within the tubular constructs was caused by a combination of gravity, fluid flow, topography, and scaffold configuration, while cell survival was influenced by scaffold length, porosity, and thickness. This research demonstrates that the mini-chambers represent a viable, novel, scale down approach for the evaluation of complex 3D scaffolds as well as providing a microbioprocessing strategy for tissue engineering and the potential repair of SCI.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Riehle, Dr Mathis and Barnett, Professor Susan and Sun, Dr Tao and Gadegaard, Professor Nikolaj and Donoghue, Dr Peter |
| Authors: | Donoghue, P. S., Sun, T., Gadegaard, N., Riehle, M. O., and Barnett, S. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Molecular Pharmaceutics |
| Publisher: | American Chemical Society |
| ISSN: | 1543-8384 |
| ISSN (Online): | 1543-8392 |
| Copyright Holders: | Copyright © 2014 The Authors |
| First Published: | First published in Molecular Pharmaceutics |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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