Morphological and cellular changes in secondary epidermal laminae of horses with insulin-induced laminitis

Karikoski, N.P., Patterson-Kane, J.C., Asplin, K.E., McGowan, T.W., McNutt, M., Singer, E.R. and McGowan, C.M. (2014) Morphological and cellular changes in secondary epidermal laminae of horses with insulin-induced laminitis. American Journal of Veterinary Research, 75(2), pp. 161-168. (doi: 10.2460/ajvr.75.2.161)

Full text not currently available from Enlighten.

Abstract

Objective—To determine cellular changes associated with secondary epidermal laminae (SEL) in forefeet and hind feet of ponies with insulin-induced laminitis.<p></p> Animals—8 ponies.<p></p> Procedures—Laminitis was induced in 4 ponies by IV administration of insulin and glucose; 4 control ponies received saline (0.9% NaCl) solution IV. Laminar tissue samples obtained from the dorsal aspects of the hooves were histologically evaluated. Primary epidermal lamina (PEL) length and width and SEL length, width, and angle were determined. Numbers of epidermal cell nuclei per micrometer and per total length of SEL and numbers of apoptotic and proliferative cells in axial, middle, and abaxial laminar regions were determined.<p></p> Results—SEL in treatment group ponies were significantly longer, were significantly narrower, and had a smaller angle relative to PEL in all laminar regions versus control ponies. In treatment group ponies, the number of epidermal cell nuclei per SEL was typically higher and the number of cells per micrometer of SEL was lower in laminar regions, apoptotic cell numbers were higher in abaxial and middle regions in forefeet and hind feet, and proliferating cell numbers were higher in axial laminar regions in forefeet and all laminar regions in hind feet, versus control ponies.<p></p> Conclusions and Clinical Relevance—Results indicated SEL elongation, narrowing, and alteration in orientation developed in all feet of ponies with insulin-induced laminitis. This was primarily attributable to cell stretching that developed at the same time as an accelerated cell death–proliferation cycle; differences in cell cycle responses among laminar regions between forefeet and hind feet may have been attributable to differences in load bearing.<p></p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Patterson-Kane, Professor Janet and McNutt, Miss Megan
Authors: Karikoski, N.P., Patterson-Kane, J.C., Asplin, K.E., McGowan, T.W., McNutt, M., Singer, E.R., and McGowan, C.M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:American Journal of Veterinary Research
ISSN:0002-9645
ISSN (Online):1943-5681

University Staff: Request a correction | Enlighten Editors: Update this record