Immunogenicity of NS4b dengue 3 virus mimotope presented to the immune system as multiple antigen peptide system

Amin, N. et al. (2013) Immunogenicity of NS4b dengue 3 virus mimotope presented to the immune system as multiple antigen peptide system. ISRN Virology, 2013, p. 924057. (doi: 10.5402/2013/924057)

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Publisher's URL: http://dx.doi.org/10.5402/2013/924057

Abstract

The availability of random peptide libraries displayed on bacteriophage (RPL) has provided a powerful tool for selecting sequences that mimic binding properties of natural antigen epitopes (mimotopes). These mimotopes can be used for vaccine design, drug development, and diagnostic assays. Several mimotopes have been shown to induce production of antibodies against the natural antigen. We have previously identified four dengue virus mimotopes from a phage-displayed peptide library using antidengue 3 human sera. Three of them showed similarity in their amino acid sequences with the NS4b proteins of dengue. Few studies have examined the role of NS4b proteins in the antibody response to dengue virus infection. A multiple antigen peptide (MAP) system was chemically synthesized containing this mimotope (NS4b MAP), and BALB/c mice were immunized to evaluate its immunogenicity. Antipeptide responses were induced and recognised DENV-3 infected cells as determined by immunofluorescence. The high levels of the IgG2a subtype against NS4bMAP suggest the induction of a Th1-like response. Our findings suggest that the NS4b mimotope might be a useful tool for the development of multiepitope diagnostic assays, dengue virus vaccine design, and pathogenesis studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stott I, Professor David
Authors: Amin, N., Pupo, M., Aguilar, A., Camacho, F., Alvarez, M., Caballero, Y., Díaz, D., García, A., Reyes, O., Guzmán, M. G., Stott, D. I., and Acosta, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:ISRN Virology
ISSN:2090-8814
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in ISRN Virology 2013:924057
Publisher Policy:Reproduced under a Creative Commons License

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