Adherence of Ureaplasma urealyticum to human epithelial cells

Smith, D.G.E., Russell, W.C. and Thirkell, D. (1994) Adherence of Ureaplasma urealyticum to human epithelial cells. Microbiology, 140(10), pp. 2893-2898. (doi:10.1099/00221287-140-10-2893)

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Adherence of Ureaplasma urealyticum cells to eukaryotic cell monolayers was quantified using the Bertholet assay to monitor ammonia produced from urea by ureaplasma urease. Adherence was abolished by pre-treatment of ureaplasmas with HeLa cell extracts and inhibited to varying degrees by pre-treatment of the ureaplasmas with N-acetylneuraminic acid, specific antisera and monoclonal antibodies. The data suggest the presence of several ureaplasma adhesins, some of which are species- or serotype-specific and some of which are proteinaceous and antigenic. The serotype-8-specific 96 kDa surface-expressed antigen may be one adhesin. Pre-treatment of HeLa cell monolayers with neuraminidase significantly reduced ureaplasma adherence and, using a novel ‘immunoblot adherence assay’, ureaplasmas were shown to bind to a number of HeLa cell components, three of which appear to terminate in sialic acid.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Smith, Professor David
Authors: Smith, D.G.E., Russell, W.C., and Thirkell, D.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Microbiology
Publisher:Society for General Microbiology
ISSN (Online):1465-2080
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