Toxoplasma gondii Syntaxin 6 is required for vesicular transport between endosomal-like compartments and the Golgi Complex

Jackson, A. J., Clucas, C., Mamczur, N. J., Ferguson, D. J. and Meissner, M. (2013) Toxoplasma gondii Syntaxin 6 is required for vesicular transport between endosomal-like compartments and the Golgi Complex. Traffic, 14(11), pp. 1166-1181. (doi:10.1111/tra.12102)

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Publisher's URL: http://dx.doi.org/10.1111/tra.12102

Abstract

Apicomplexans are obligate intracellular parasites that invade the host cell in an active process that relies on unique secretory organelles (micronemes, rhoptries and dense granules) localized at the apical tip of these highly polarized eukaryotes. In order for the contents of these specialized organelles to reach their final destination, these proteins are sorted post-Golgi and it has been speculated that they pass through endosomal-like compartments (ELCs), where they undergo maturation. Here, we characterize a Toxoplasma gondii homologue of Syntaxin 6 (TgStx6), a well-established marker for the early endosomes and trans Golgi network (TGN) in diverse eukaryotes. Indeed, TgStx6 appears to have a role in the retrograde transport between ELCs, the TGN and the Golgi, because overexpression of TgStx6 results in the development of abnormally shaped parasites with expanded ELCs, a fragmented Golgi and a defect in inner membrane complex maturation. Interestingly, other organelles such as the micronemes, rhoptries and the apicoplast are not affected, establishing the TGN as a major sorting compartment where several transport pathways intersect. It therefore appears thatToxoplasma has retained a plant-like secretory pathway.

Item Type:Articles (Other)
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus and Mamczur, Miss Nicola and Jackson, Dr Allison and Clucas, Dr Caroline
Authors: Jackson, A. J., Clucas, C., Mamczur, N. J., Ferguson, D. J., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Research Group:Markus Meissner
Journal Name:Traffic
Publisher:Wiley
ISSN:1398-9219
ISSN (Online):1600-0854
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in Traffic 14(11):1166-1181
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
501601Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY
501602Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY