Dominant role of nucleotide substitution in the diversification of serotype 3 pneumococci over decades and during a single infection

Croucher, N.J. et al. (2013) Dominant role of nucleotide substitution in the diversification of serotype 3 pneumococci over decades and during a single infection. PLoS Genetics, 9(10), e1003868. (doi: 10.1371/journal.pgen.1003868)

[img]
Preview
Text
92056.pdf - Published Version
Available under License Creative Commons Attribution.

1MB

Abstract

Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis of a complete reference genome and 81 draft sequences from clonal complex 180, the predominant serotype 3 clone in much of the world, found most sampled isolates belonged to a clade affected by few diversifying recombinations. However, other isolates indicate significant genetic variation has accumulated over the clonal complex's entire history. Two closely related genomes, one from the blood and another from the cerebrospinal fluid, were obtained from a patient with meningitis. The pair differed in their behaviour in a mouse model of disease and in their susceptibility to antimicrobials, with at least some of these changes attributable to a mutation that up-regulated the patAB efflux pump. This indicates clinically important phenotypic variation can accumulate rapidly through small alterations to the genotype.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Inverarity, Dr Donald
Authors: Croucher, N.J., Mitchell, A.M., Gould, K.A., Inverarity, D., Barquist, L., Feltwell, T., Fookes, M.C., Harris, S.R., Dordel, J., Salter, S.J., Browall, S., Zemlickova, H., Parkhill, J., Normark, S., Henriques-Normark, B., Hinds, J., Mitchell, T.J., and Bentley, S.D.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:PLoS Genetics
Publisher:Public Library of Science
ISSN:1553-7390
ISSN (Online):1553-7390
Copyright Holders:Copyright © 2013 The Authors
First Published:First published in PLoS Genetics 9(10):e1003868
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record