Use of a continuous culture system linked to a modified robbins device or flow cell to study attachment of bacteria to surfaces

Millar, M. R., Linton, C. J. and Sherriff, A. (2001) Use of a continuous culture system linked to a modified robbins device or flow cell to study attachment of bacteria to surfaces. Methods in Enzymology, 337, pp. 43-62. (doi:10.1016/S0076-6879(01)37005-2)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1016/S0076-6879(01)37005-2

Abstract

This chapter describes the use of a continuous culture system linked to a modified Robbins device (MRD) to compare the adhesion of bacteria grown under defined conditions to different surfaces. The chemostat allows the numbers, growth conditions, and growth rates of the bacteria used in adhesion experiments to be controlled. Use of a chemostat allows conditions that promote adhesion and/or biofilm formation to be selected. The chapter uses this system to study the impact of shear stress and flow rate, changing ionic environment, and substrate properties on adhesion of Staphylococcus epidermidis RP 62A. In principle the methods could be used to select for bacterial phenotypes or genotypes with preferential adhesion to different surfaces using mono- or polymicrobial cultures. The MRD has been used predominantly for biofilm studies rather than for adhesion studies. In experiments, particularly at low flow rates, there was a trend toward higher numbers adhering to the discs at the in-flow end of the MRD than at the outflow end, probably reflecting depletion of adherent bacteria in the interacting stream. The chapter also describes a mathematical simulation model of the experimental system. Other representations may yield better agreement with experimental results. How statistical modeling tools can aid in the understanding of complex systems, which cannot always be formulated and solved analytically have been demonstrated.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sherriff, Dr Andrea
Authors: Millar, M. R., Linton, C. J., and Sherriff, A.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Methods in Enzymology
Publisher:Elsevier
ISSN:0076-6879
ISSN (Online):1557-7988
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record