The CRF-related peptide sauvagine stimulates and the GABAB receptor agonist baclofen inhibits cyclic-AMP production in melanotrope cells of Xenopus laevis

Jenks, B.G., van Zoest, I.D., De Koning, H.P. , Leenders, H.J. and Roubos, E.W. (1991) The CRF-related peptide sauvagine stimulates and the GABAB receptor agonist baclofen inhibits cyclic-AMP production in melanotrope cells of Xenopus laevis. Life Sciences, 48(17), pp. 1633-1637. (doi:10.1016/0024-3205(91)90123-S)

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Publisher's URL: http://dx.doi.org/10.1016/0024-3205(91)90123-S

Abstract

Release of α-MSH from the pars intermedia melanotrope cells of Xenopus laevis is regulated by various classical neurotransmitters and neuropeptides. We have examined the effect of two of these regulatory substances, the neurotransmitter GABA and the CRF-related peptide sauvagine, on the adenylate cyclase system of the melanotrope cells. Sauvagine treatment, which stimulates α-MSH release, lead to an elevation in the level of cyclic-AMP, an effect which was potentiated by cholera toxin. Treatment with baclofen, a GABAB receptor agonist, gave a pertussis toxin-sensitive decrease in the cyclic-AMP level and an inhibition of α-MSH release. We conclude that sauvagine stimulates α-MSH secretion through activation of adenylate cyclase and that GABAB receptor activation inhibits secretion through inhibition of cyclic-AMP production. Baclofen treatment sensitized melanotrope cells to the stimulatory action of 8-bromo-cyclic-AMP on the secretion of α-MSH. This observation supports the conclusion that GABAB receptor activation inhibits cyclic-AMP production.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:De Koning, Professor Harry
Authors: Jenks, B.G., van Zoest, I.D., De Koning, H.P., Leenders, H.J., and Roubos, E.W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Life Sciences
Publisher:Elsevier
ISSN:0024-3205
ISSN (Online):1879-0631

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