The streamlined genome of Phytomonas spp. Relative to human pathogenic kinetoplastids reveals a parasite tailored for plants

Porcel, B. M. et al. (2014) The streamlined genome of Phytomonas spp. Relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. PLoS Genetics, 10(2), e1004007. (doi:10.1371/journal.pgen.1004007)

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Publisher's URL: http://dx.doi.org/10.1371/journal.pgen.1004007

Abstract

Members of the family Trypanosomatidae infect many organisms, including animals, plants and humans. Plant-infecting trypanosomes are grouped under the single genus Phytomonas, failing to reflect the wide biological and pathological diversity of these protists. While some Phytomonas spp. multiply in the latex of plants, or in fruit or seeds without apparent pathogenicity, others colonize the phloem sap and afflict plants of substantial economic value, including the coffee tree, coconut and oil palms. Plant trypanosomes have not been studied extensively at the genome level, a major gap in understanding and controlling pathogenesis. We describe the genome sequences of two plant trypanosomatids, one pathogenic isolate from a Guianan coconut and one non-symptomatic isolate from Euphorbia collected in France. Although these parasites have extremely distinct pathogenic impacts, very few genes are unique to either, with the vast majority of genes shared by both isolates. Significantly, both Phytomonas spp. genomes consist essentially of single copy genes for the bulk of their metabolic enzymes, whereas other trypanosomatids e.g. Leishmania and Trypanosoma possess multiple paralogous genes or families. Indeed, comparison with other trypanosomatid genomes revealed a highly streamlined genome, encoding for a minimized metabolic system while conserving the major pathways, and with retention of a full complement of endomembrane organelles, but with no evidence for functional complexity. Identification of the metabolic genes of Phytomonas provides opportunities for establishing in vitro culturing of these fastidious parasites and new tools for the control of agricultural plant disease.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dickens, Dr Nicholas and Hammarton, Dr Tansy and Mottram, Professor Jeremy
Authors: Porcel, B. M., Denoeud, F., Opperdoes, F., Noel, B., Madoui, M.-A., Hammarton, T. C., Field, M. C., Da Silva, C., Couloux, A., Poulain, J., Katinka, M., Jabbari, K., Aury, J.-M., Campbell, D. A., Cintron, R., Dickens, N. J., Docampo, R., Sturm, N. R., Koumandou, V. L., Fabre, S., Flegontov, P., Lukeš, J., Michaeli, S., Mottram, J. C., Szöőr, B., Zilberstein, D., Bringaud, F., Wincker, P., and Dollet, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS Genetics
Publisher:Public Library of Science
ISSN:1553-7390
ISSN (Online):1553-7390
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in PLoS Genetics 10(2):e1004007
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
371796The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/Z/08/ZIII - PARASITOLOGY
454141Analysing the roles of petidases in Leishmania infectivity and pathogenicityJeremy MottramMedical Research Council (MRC)G0700127III - PARASITOLOGY