The Toxoplasma Acto-MyoA motor complex is important but not essential for gliding motility and host cell invasion

Egarter, S., Andenmatten, N., Jackson, A. J., Whitelaw, J. A., Pall, G., Black, J. A., Ferguson, D. J.P., Tardieux, I., Mogilner, A. and Meissner, M. (2014) The Toxoplasma Acto-MyoA motor complex is important but not essential for gliding motility and host cell invasion. PLoS ONE, 9(3), e91819. (doi:10.1371/journal.pone.0091819) (PMID:24632839) (PMCID:PMC3954763)

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Publisher's URL: http://dx.doi.org/10.1371/journal.pone.0091819

Abstract

Apicomplexan parasites are thought to actively invade the host cell by gliding motility. This movement is powered by the parasite own actomyosin system and depends on the regulated polymerisation and depolymerisation of actin to generate the force for gliding and host cell penetration. Recent studies demonstrated that Toxoplasma gondii can invade the host cell in the absence of several core components of the invasion machinery, such as the motor protein myosin A (MyoA), the microneme proteins MIC2 and AMA1 and actin, indicating the presence of alternative invasion mechanisms. Here the roles of MyoA, MLC1, GAP45 and Act1, core components of the gliding machinery, are re-dissected in detail. Although important roles of these components for gliding motility and host cell invasion are identified, mutant parasites remain invasive and do not show a block of gliding motility, demonstrating that other mechanisms must be in place to enable the parasite to move and invade the host cell. A novel model for parasite gliding motility and invasion is presented based on hydrodynamic forces generated in the cytosol of the parasite that are converted into motility.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus and Andenmatten, Ms Nicole and Pall, Dr Gurman and Jackson, Dr Allison
Authors: Egarter, S., Andenmatten, N., Jackson, A. J., Whitelaw, J. A., Pall, G., Black, J. A., Ferguson, D. J.P., Tardieux, I., Mogilner, A., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in PLoS ONE 9(3):e91819
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
371796The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/Z/08/ZIII - PARASITOLOGY
501602Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY